GHK

Regenerative Tripeptideresearch

Also known as: Glycyl-L-Histidyl-L-Lysine, GHK Tripeptide, Liver Cell Growth Factor Fragment

A naturally occurring tripeptide that resets gene expression to a younger state, promoting wound healing, collagen synthesis, and anti-inflammatory effects — the copper-free precursor to GHK-Cu.

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Overview

GHK (Glycyl-Histidyl-Lysine) is a naturally occurring tripeptide first isolated from human plasma in 1973. It was discovered as a factor in young human plasma that could restore the protein synthesis of aged liver tissue to a younger state. GHK is the base peptide that, when complexed with copper(II), forms GHK-Cu. However, GHK itself — without copper — has significant biological activity, particularly in gene expression regulation. Microarray studies have shown that GHK can modulate the expression of over 4,000 genes, resetting many to a pattern associated with younger tissue. It upregulates genes involved in wound healing, collagen synthesis, and anti-inflammatory responses while suppressing genes associated with inflammation, tissue destruction, and cancer metastasis. GHK is used both in cosmetics (topical) and as a research peptide for its gene-modulatory and tissue-repair properties.

Mechanism of Action

GHK modulates gene expression and tissue repair: (1) Resets expression of 4,000+ human genes toward a healthier, younger pattern; (2) Upregulates collagen I, III, and IV gene expression in fibroblasts; (3) Stimulates decorin and other proteoglycan production; (4) Suppresses fibrinogen and inflammatory gene networks; (5) Activates ubiquitin-proteasome system for removal of damaged proteins; (6) Upregulates DNA repair genes; (7) Suppresses genes associated with metastasis (CEACAM1, LYPD3); (8) Has intrinsic copper-binding affinity that can chelate and deliver trace copper to tissues.

Molecular Formula

C14H24N6O4

Molecular Weight

340.38 g/mol

Sequence

Gly-His-Lys

Dosage Protocols

Dose Range

0.01% concentration0.1% concentration

Frequency

Twice daily

Route

topical

Cycle Length

Ongoing

Applied to face and neck. Can be formulated with or without copper complexation. Results visible after 8-12 weeks of consistent use.

Source: Cosmeceutical studies

🧮 Personalized Dosage Calculator

💰 Estimated Pricing

$20 – $60per vialresearch

Typical Supply

50mg vial

Last Updated

2026-02

Base tripeptide without copper. Less common than GHK-Cu. Research vendor pricing.

⚠️ Prices are estimates based on publicly available data and may vary significantly by vendor, location, and prescription status. This is not medical or financial advice.

Side Effects

EffectSeverity
Mild skin irritation (topical)mild
Injection site reactionmild

Pros & Cons

Modulates 4,000+ genes toward a younger expression pattern — broad anti-aging effects

Natural human peptide with excellent safety profile and decades of research

Active both topically and systemically

Suppresses cancer-related gene networks while promoting repair genes

Less potent than GHK-Cu for wound healing and many applications

Small peptide with relatively short half-life in circulation

Gene expression changes take time — not fast-acting

Research Studies

🩸 Blood Work

No specific bloodwork requirements reported for this peptide. General health panels are always recommended before starting any peptide protocol.

Legal Status

Commercially available as a cosmetic ingredient and research chemical. No prescription required. No regulatory restrictions. Not a drug in most jurisdictions.

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